Biopro hic bf
WebApr 30, 2024 · The best HIC column for each separation: BioPro HIC HT and BioPro HIC BF; Analysis of commercial monoclonal antibodies by microLC-MS/MS; Opportunities and challenges in the booming North American cannabis market; 5 Days of Exciting Science and Innovative Talks by Experts in the World of Liquid Phase Separation Science and Analysis WebColumn: BioPro HIC BF (4 µm) 100 x 4.6 mm ID Part number: BHB00S04-1046WT Eluent: A) 3 M ammonium acetate in water B) 100 % water Gradei nt: 0 % B 0( – 2 mni ) 0 – 90 % B 2( – 18 mni ) 90 % B 1(8 – 22 mni ) Flow rate: 0.3 mL/min Sample: Mixture of 7 in-house MAbs at 1 – 2 mg/mL each 2 in-house MAbs with molecular variants
Biopro hic bf
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WebThe YMC BioPro HIC BF is a hydrophobic interaction chromatography (HIC) column utilizing a butyl (C4) stationary phase bonded to non-porous hydrophilic polymer particles. This HIC phase is specifically designed for fast, high resolution separations of proteins and biopharmaceutical drugs such as monoclonal antibodies (mAb’s), including drug ... WebBioPro HIC BF: Resin: Hydrophillic non-porous polymer beads: Ligand: Butyl: Particle Size: 4 µm: Column Size: 100 x 4.6 mm: Recommended Flow Rate: 0.5 - 1 mL/min: Maximum …
WebMar 31, 2024 · HIC is a reference method to separate the hydrophobic variants of protein species. In most HIC separations, the same (historical) conditions are usually applied (such as a 1.5–2 M ammonium‐sulfate buffer at pH 6–7 for the mobile phase with a butyl stationary phase), although many other options and conditions can be used that could improve the … WebReaction monitoring of Antibody-Drug-Conjugates (ADCs) using YMC´s BioPro HIC BF: Antibody Drug Conjugate (RIKEN) BHB00S04-1046WT: BioPro HIC BF: View. Download. Antibodies: Generic Analyses of Different Monoclonal Antibodies with RP-(U)HPLC:
WebBioPro HIC BF shows the stronger retention of proteins due to the higher hydrophobicity of its stationary phase compared to the other … WebBioPro HIC BF. Base particle: Hydrophilic polymer (polymethacrylate) Particle size: 4 µm: Pore size: Non-porous: Functional group: Butyl: pH range: 2.0 - 12.0: Temperature …
WebMicro-flow reaction (Flow reactor) is a new solution performing chemical reaction by delivering raw materials into micro channels. Flow reactor enables various types of reactions which could not have been performed in a conventional batch mode (flask, vessel). YMC offers KeyChem series for flow reaction. KeyChem is an abbreviation for keyboard ...
WebBioPro HIC BF with 4 µm particle size and a competitor’s column with 2.5 µm particles. BioPro HIC BF, with its optimized media and packing technology, shows superior peak shape and resolution that compare favorably with the competitor’s sub-3 µm column. BioPro HIC BF (4 ˜m) Competitor HIC column (2.5 ˜m) 3.3-4.6 MPa 7.3-10.5 MPa Rs (1 ... french king charles viiiWebSep 9, 2024 · As BioPro HIC HT is designed for the analysis of biopharmaceuticals, it is the first choice for ADCs or MAbs. It provides high resolution with very fast runs and therefore high throughput. BioPro HIC … french king cake recipe authenticWebOct 26, 2024 · Use of 2 different YMC HIC columns. YMC’s BioPro HIC BF and BioPro HIC HT were used in order to determine the effect on intact protein retention. The proteins used for evaluation were cytochrome c, myoglobin, ribonuclease A, lysozyme, carbonic anhydrase, trypsinogen and α-chymotrypsinogen A in varying combinations. french king charles iiWebSep 19, 2024 · This application note explores when to use each of YMC’s two HIC columns by comparing retention behaviors of BioPro HIC HT and BioPro HIC BF. Search. Spotlight. High-Throughput Analysis of Volatile ... fasthosts terms and conditionsWebSep 9, 2024 · As BioPro HIC HT is designed for the analysis of biopharmaceuticals, it is the first choice for ADCs or MAbs. It provides high resolution with very fast runs and … fasthosts transfer domainWebThe right HIC column for each separation- BioPro HIC HT and BioPro HIC BF: Application Note: EN: Download. Charakterisierung von monoklonalen Antikörpern und verwandten Produkten via HIC-MS: Product Information: DE: Download. Characterisation of MAbs and related products via HIC-MS: Product Information: EN: french kingdomWebBioPro HIC BF Perfect choice for DAR! Effective for Drug-to-Antibody Ratio (DAR) Analysis of ADCs Sample: Cystein-conjugated ADC mimic (1.25 mg/mL) Specifications Base … fasthosts unlock domain