Pherd20t
WebPseudomonas aeruginosa is a widely distributed non-fermentative Gram-negative opportunistic pathogen that is often responsible for nosocomial infections. Gene … WebPlasmid pUCP20T-ecfp from Dr. Mariette Barbier's lab contains the insert eCFP and is published in PLoS One. 2016 Mar 3;11(3):e0146827. doi: 10.1371/journal.pone.0146827. …
Pherd20t
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Web7. okt 2024 · The resultant pHERD20T-rpoS plasmid was used as template to amplify a 2376 bp DNA fragment with the oligonucleotides araCTHindIIIFw and rpoSHindIIIRv. The PCR … WebThe sspA and sspB genes from P . aeruginosa were cloned behind the P BAD promoter in the pHERD20T vector, and conjugated into CF149. (A) Western blot analysis of SspA and …
http://www.biofeng.com/zaiti/dachang/pet15b.html Web13. apr 2024 · .本发明属于基因突变技术领域，尤其是一种新型转座子突变株文库的构建系统、新型转座子突变文库和应用。背景技术.转座子(tn)，亦称跳跃基因，是指能将自身基因 …
http://www.biovector.net/product/163198.html Web22. dec 2024 · Patients and methods: The shuttle vector pHERD20T containing an arabinose inducible promoter was used to construct the CRISPRi system. Alterations in cellular …
Web21. dec 2024 · This allows for an efficient transfer of the phage ORFan gene from the already available pENTR/SD/D-TOPO_ORFan plasmid (Subheading 3.1.1) into the Gateway …
Web1. okt 2024 · PAO1 harbouring pHERD20T‐dCas9 or pHERD20T‐dCas9‐prtR were used in the tests. (d) Plating efficiencies of 5 × 10 8 /ml bacterial cells (PAO1 harbouring … book creator download windows deutschWeb28. apr 2024 · PA14 harbouring pHERD20T-tclT was >4-fold more resistant to TC + DSX compared with the empty vector control (Figure 5c), while the same strain expressing P. … book creator download freeA previously described31 environmental strain of P. aeruginosa was used as a template to amplify the four cas genes of the Type I-C CRISPR–Cas system genes (cas3, cas5, cas7 and cas8). The genes were cloned into … Zobraziť viac Transformations of P. aeruginosa, E. coli, P. syringae and K. pneumoniae strains were conducted using standard electroporation … Zobraziť viac PAO1IC was grown overnight at 37 °C in LB media. Then 150 μl of overnight culture was added to 4 ml of 0.7% LB top agar and spread on 1.5% LB agar plates supplemented … Zobraziť viac The isogenic DMS3macrIIA4 and acrIC1 phages were constructed using previously described methods60. A recombination cassette, pJZ01, was constructed with homology to the … Zobraziť viac book creator ecoleWebEscherichia-Pseudomonas shuttle vector pHERD20T. Disclaimer: The NCBI taxonomy database is not an authoritative source for nomenclature or classification - please consult … god of war 1.0.13Web6. dec 2024 · The highest PYO production was found in ΔpilA/pHerd20T, which showed low bioelectricity output (half of PAO1/pHerd20T). In ΔpilA or PAO1, expression of either … book creator educationWeb(A) AlgUA61V retained the function of inducing alginate production. The wild type algU gene of PAO1 and its variant of CF149 were cloned into pHERD20T, and conjugated into … book creator ebookWebNational Center for Biotechnology Information god of war 100 walkthrough neo